Gene Regulation in N Mutants of Bacteriophage λ
نویسندگان
چکیده
منابع مشابه
Regulation of bacteriophage P2 late-gene expression: the ogr gene.
The ogr gene product of bacteriophage P2 is a positive regulatory factor required for P2 late-gene transcription. We have determined the nucleotide sequence of the ogr gene, which encodes a basic polypeptide of 72 amino acids. P2 growth is blocked by a host mutation, rpoA109, in the alpha subunit of DNA-dependent RNA polymerase. The ogr52 mutation, which allows P2 to grow in an rpoA109 strain, ...
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HE apparent mutation rate depends upon a number of contingencies: the Tprimary error rate, the probability o€ repair, error avoidance by the apparatus of DNA replication, and the probability of detection. The probability of detection of those base pair substitutions which produce an amino acid substitution appears to be highly variable, and presumably reflects the sensitivity of polypeptide fun...
متن کاملWhy does the positive regulation in bacteriophage λ affect isomerization of the RNAP-DNA complex?
The initiation of transcription involves three steps: binding, opening, and escape of RNA polymerase. To model these steps in the simplest way we will treat opening and escape as a single reaction with forward reaction rate k determined by the regulatory proteins and their interaction with the DNA. Binding will be treated as a reversible reaction with an equilibrium constant KB. Using mathemati...
متن کاملProperties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.
In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled wi...
متن کاملBacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
Bacterial mutants have been isolated, called groN, that block phage development by interference with the action of the product of the phage N gene. lambdatrp phages, which depend on the N product for the synthesis of tryptophan enzymes, do not make these enzymes in groN bacteria. Two type of phage mutants have been isolated that can overcome the groN block. One type makes an altered N product, ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.9.6.938-945.1972